Can be used simultaneously with ScriptCap m 7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.įor post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produceĬap 1 caps, that which is found on most eukaryotic mRNAs.įor conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. This cannot be accomplished with co-transcriptional capping methods. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours.įor post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.įor the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA inįor the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. See T7-FlashScribe Transcription Kit, ScriptCap m 7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page.įor the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. Simply the best way to make in vitro mRNA. T7 mScript™ Standard mRNA Production System

Scribe kit